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BIOlabSem 6

DOWNSTREAM PROCESSING AND BIOPROCESS ENGINEERING LAB

BIO 3142

Syllabus

  • 01aqueous two phase based separation
  • 02extraction of intracellular proteins
  • 03precipitation of proteins
  • 04ultrafiltration
  • 05separation of solids from slurry using leaf filter and sedimentation
  • 06estimation of enzyme kinetics, optimum temperature, pH and evaluation of the type of inhibition during enzyme catalyzed reactions

References

  • Cussler, E. L., et al. Bioseparations : downstream processing for biotechnology. United Kingdom, Wiley, 1988.
  • Asenjo, Juan A.. Separation Processes in Biotechnology. United States, CRC Press, 2020.
  • Michael L Shuler and FikretKargi. Bioprocess Engineering: Basic Concepts. Prentice-Hall of India Pvt Ltd 2008.
Credits Structure
0Lecture
0Tutorial
6Practical
2Total